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KMID : 0386319910240010086
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Korean Leprosy Bulletin
1991 Volume.24 No. 1 p.86 ~ p.89
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Detection of Mycobacterium leprae in Skin Smear Samples from Leprosy Patients by the Polymerase Chain Reaction
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성경제/Kyung Jea Sung
서호석/최지호/고재경/나도선/정구원/Ho Suk Suh/Jee Ho Choi/Jae Kyung Ko/Do Sun Na/Koo Won Jung
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Abstract
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The development of polymerase chain reaction (PCR) technique to detect
Mycobacterium leprae using specific DNA sequence is a major advance in early,
definitive diagnosis of leprosy. By using the same primer and PCR condition that we
preformed used in PCR of fresh-frozen and paraffin-embedded tissues from leprosy
patients, we peformed PCR in skin smear samples from 11 leprosy patients. This
procedure amplified a 360-base-pair product from M. leprae genomic DNA in 4 out of 8
multibacillary leprosy patients but DNA templates from 3 paucibacillary leprosy patients
were not amplified. Therefore, skin smear samples may be inadequate in performing
PCR for M. leprae DNA. We discussed the reasons of the discrepancy between the
positive rate of PG using paraffin-embedded sections and that in skin smear samples. .
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